Role of C/EBP-β in Methamphetamine-Mediated Microglial Apoptosis

Chen, Xuebing and Lu, Jiancong and Zhao, Xu and Chen, Chuanxiang and Qiao, Dongfang and Wang, Huijun and Yue, Xia (2019) Role of C/EBP-β in Methamphetamine-Mediated Microglial Apoptosis. Frontiers in Cellular Neuroscience, 13. ISSN 1662-5102

[thumbnail of pubmed-zip/versions/1/package-entries/fncel-13-00366/fncel-13-00366.pdf] Text
pubmed-zip/versions/1/package-entries/fncel-13-00366/fncel-13-00366.pdf - Published Version

Download (5MB)

Abstract

Methamphetamine (MA) is a widely abused psychoactive drug that primarily damages the nervous system. However, the involvement of MA in the survival of microglia remains poorly understood. CCAAT-enhancer binding protein (C/EBP-β) is a transcription factor and an important regulator of cell apoptosis. Lipocalin2 (lcn2) is a known apoptosis inducer and is involved in many cell death processes. We hypothesized that C/EBP-β is involved in MA-induced lcn2-mediated microglial apoptosis. To test this hypothesis, we measured the protein expression of C/EBP-β after MA treatment and evaluated the effects of silencing C/EBP-β or lcn2 on MA-induced apoptosis in BV-2 cells and the mouse striatum after intrastriatal MA injection. MA exposure increased the expression of C/EBP-β and stimulated the lcn2-mediated modulation of apoptosis. Moreover, silencing the C/EBP-β-dependent lcn2 upregulation reversed the MA-induced microglial apoptosis. The in vivo relevance of these findings was confirmed in mouse models, which demonstrated that the microinjection of anti-C/EBP-β into the striatum ameliorated the MA-induced decrease survival of microglia. These findings provide a new insight regarding the specific contributions of C/EBP-β-lcn2 to microglial survival in the context of MA abuse.

Item Type: Article
Subjects: STM Digital > Medical Science
Depositing User: Unnamed user with email support@stmdigital.org
Date Deposited: 26 May 2023 07:07
Last Modified: 16 Sep 2024 10:38
URI: http://research.asianarticleeprint.com/id/eprint/944

Actions (login required)

View Item
View Item