The Effect of KIN and 2,4-D on In vitro Propagation of Garlic (Allium sativum L.)

The research work was conducted to observe in vitro propagation of garlic in the Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Bangladesh from the period of July 2015 to June, 2016, to determine the effect of Kinetin (KIN) and 2, 4-Dichlorophenoxy acetic acid (2, 4-D) in Murashige and Skoog (MS) medium where disease free, healthy and sterilized basal part of clove (2-3 cm) was used as explants. The highest shoot regeneration percentage (100%) was obtained from the treatment of 3.0 mg/L of KIN alone and also from all combination of 2.5, 3.0 mg/L KIN with 1.0, 1.5, 2.0, 2.5 mg/L 2, 4-D. All treatments of KIN and 2, 4-D either alone or in combination, showed one number of shoot after 3 weeks of inoculation. The highest shoot length (34.50 cm) was observed in 3.0 mg/L KIN treatment whereas in combined treatments, 3.0 mg/L KIN+ 2.0 mg/L 2, 4-D showed highest 33.73 cm shoot length after 3 weeks from inoculation. Besides, maximum root induction percentage (100%) was observed by 2.5 mg/L KIN + (1.0, 1.5) mg/L 2, 4-D. The highest number of roots (10.67) were developed by 3.0 mg/L KIN+ 1.5 mg/L 2, 4-D. After transferring the plantlets in the field condition, 86% survival plants were recorded. Finally, the in vitro regeneration protocol of garlic described herein can potentially be used as a tool in molecular breeding programs for improvement of different cultivars and genotypes of garlic.