Placenta mesenchymal stem cells differentiation toward neuronal-like cells on nanofibrous scaffold

Rahimi-Sherbaf, Fatemeh and Nadri, Samad and Rahmani, Ali and Dabiri Oskoei, Atousa (2020) Placenta mesenchymal stem cells differentiation toward neuronal-like cells on nanofibrous scaffold. BioImpacts, 10 (2). pp. 117-122. ISSN 2228-5660

[thumbnail of Unconfirmed 87927.crdownload] Text
Unconfirmed 87927.crdownload - Published Version

Download (1MB)

Abstract

Introduction: Transplantation of stem cells with a nanofibrous scaffold is a promising approach for spinal cord injury therapy. The aim of this work was to differentiate neural-like cells from placenta-derived mesenchymal stem cells (PDMSCs) using suitable induction reagents in three (3D) and two dimensional (2D) culture systems.
Methods: After isolation and characterization of PDMSCs, the cells were cultivated on poly-L-lactide acid (PLLA)/poly caprolactone (PCL) nanofibrous scaffold and treated with a neuronal medium for 7 days. Electron microscopy, qPCR, and immunostaining were used to examine the differentiation of PDMSCs (on scaffold and tissue culture polystyrene [TCPS]) and the expression rate of neuronal markers (beta-tubulin, nestin, GFAP, and MAP-2).
Results: qPCR analysis showed that beta-tubulin (1.672 fold; P ≤ 0.0001), nestin (11.145 fold; P ≤ 0.0001), and GFAP (80.171; P ≤ 0.0001) gene expressions were higher on scaffolds compared with TCPS. Immunofluorescence analysis showed that nestin and beta-tubulin proteins were recognized in the PDMSCs differentiated on TCPS and scaffold after 7 days in the neuroinductive differentiation medium.
Conclusion: Taken together, these results delegated that PDMSCs differentiated on PLLA/PCL scaffolds are more likely to differentiate towards diversity lineages of neural cells. It proposed that PDMSCs have cell subpopulations that have the capability to be differentiated into neurogenic cells.

Item Type: Article
Subjects: STM Digital > Medical Science
Depositing User: Unnamed user with email support@stmdigital.org
Date Deposited: 31 Mar 2023 07:29
Last Modified: 30 Jul 2024 14:10
URI: http://research.asianarticleeprint.com/id/eprint/460

Actions (login required)

View Item
View Item