Optimization of Plant Growth Regulators in Indirect Plant Regeneration in Rice Variety Nerica 3 Using Mature Seeds as Explant

The study is the first attempt to highlight about callus induction and plantlet regeneration in rice variety ‘Nerica 3’ using mature seed as explant and using five regeneration media with plant growth regulators (BA, IAA, and 2,4-D) at different concentrations in other to establish the efficient indirect plant regeneration protocol. Among the five regeneration media tested, R3 medium (2 mg/L BA), showed greater percentage of plantlet regeneration (45.31%) followed by R2 medium (0.5 mg/L BA + 0,1 mg/L IAA; 29.68%), whereas, R4 medium showed the lowest (2 mg/L 2,4-D; 0%). These results will be used for genetic improvement programs of the variety ‘Nerica 3’.