EFFECT OF KOREAN WINE WASTE EXTRACTION ON NITRIC OXIDE AND CYTOKINE PRODUCTION IN LIPOPOLYSACCHARIDE-INDUCED INFLAMMATION

The effect of extract of Korean wine waste on the production of nitric oxide (NO) and cytokines in splenocytes and
RAW 264.7 cells was investigated using lipopolysaccharide (LPS) as a stimulant. Treatment of RAW 264.7 cells
with the 85% aqueous methanol (MeOH) fraction from Korean wine waste reduced LPS-induced NO production in a
dose-dependent manner (p<0.05). Culture supernatants of splenocytes exposed to a co-treatment of 85% aq.
MeOH fraction and LPS were harvested to determine the production levels of cytokines [IL-2, IL-4, IL-5, IL-6, IL-
12/IL-23(p40) and IFN-γ]. As measured by ELISA, co-administration of 85% aq. MeOH fraction (10 μg/mL) with LPS
significantly reduced the IL-2 expression after 48 h of treatment, as compared to treatment with only LPS (p<0.05),
where the level of IL-6 expression was significantly lower after 24 h of the co-treatment (p<0.05). The IL-12/IL-
23(p40) expression levels after 24 and 72 h of co-treatment were also significantly lower than that from LPS
treatment only (p<0.05). However, the IL-4 and IFN-γ expression levels were significantly lower only after 72 h of co-treatment. These results indicate that the 85% aq. MeOH fraction from Korean wine waste decreased the
production of NO as well as the expression of the pro-inflammatory cytokines IL-2, IL-6 and IL-12/IL-23(p40).